movie file size 7.69MB |
Numerical simulation of flow of a single neutrophil through
a capillary segment which is simplified to an axisymmetric
arc-shaped constriction in a straight pipe.
The neutrophil is assumed to be a sphere of a Maxwell material
encapsulated by a thin elastic membrane with a cortical tension.
The cell is driven by a constant pressure difference applied
between inlet and outlet of the capillary segment.
A mathematical model to predict transit time of a cell through
a capillary segment is developed based on the numerical result. Numerical simulation is performed with a commercial finite element solver, ADINA. |
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movie file size 19.45MB |
Cross section of capillaries is not a perfect circle.
Travel of a neutrophil rhtough a rectangular channel is simulated
and compared with that through the axisymmetric model for the
investigation of influence of the cross-sectional shape of the
capillary on the transit time of the cell through the segment. Numerical simulation is performed with a commercial finite element solver, ADINA. |
movie file size 37.20MB |
Flow of a neutrophil through a microchannel made of PDMS is observed for the comparison with the numerical simulation. The neutrophil is retained at the constriction as shown in the numerical simulation. |
movie file size 86.05MB |
The mathematical model afore mentioned is integrated to the
simulation model of flow of neutrophils in a capillary network.
Black circles in the left-hand-side figure are the neutrophils.
A constant pressure difference is applied between inlet (lower
left) and outlet (upper right) of the network to drive the cells.
A neutrophil plugging changes flow pattern of plasma in the
network to influence flow of other cells. Numerical simulation is performed with MATLAB. |
movie file size 60.01MB |
It can be thought that friction of neutrophils on endothelium of capillaries can influence transit characteristics of the cells in the capillaries. Moreover, neutrophils tend to adhere to the nedothelium different from erythrocytes. Adhesion or friction force of neutrophils on various flat plate is observed using inclined centrifugal microscope. |